Abstract
Neurodegenerative disorders results in inflammatory processes, including inflammatory cytokine secretion and concomitant superoxide production. Acetylcholinesterase inhibitors (AChEIs) influence neuro degeneration through anti-inflammatory effects. Donepezil as an AChEIs also provide neuro protection. This study aimed to evaluate the effect of donepezil (DZ) as a potent acetyl-cholinesterase (AChE) inhibitor, L-N-G-Nitroarginine methyl ester (L. NAME) as non-selective nitric oxide synthase inhibitor and 7-nitroindazole (7-NI) as a selective neuronal nitric oxide synthase (NOS) inhibitor against Amyloid beta-peptide (1-42) (A beta((1-42))) induced neurological disorder. Rats were divided into six groups including control, A beta((1-42)), A beta((1-42)) +L-arginine, A beta((1-42)) +L-NAME, A beta((1-42)) +7-NI, and A beta((1-42)) +DZ. Brain AChE, malondialdehyde, nitric oxide, super oxidedismutase, catalase, reduced glutathione, interleukin 1-beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) were measured. Also, brain fatty acids fractions were estimated by high performance liquid chromatography (HPLC). A beta((1-42)) significantly alter levels of brain antioxidant, inflammatory markers and fatty acids content compared to the control group. Treatment with L. NAME, 7-NI and especially DZ improve these parameters. Administration of acetylcholinesterase inhibitors such as DZ attenuates neurodegenerative disorders through decreasing oxidation and inflammation.