An assessment of histone-modification antibody quality

Thea A. Egelhofer; Aki Minoda; Sarit Klugman; Kyungjoon Lee; Paulina Kolasinska-Zwierz; Artyom A. Alekseyenko; Ming-Sin Cheung; Daniel S. Day; Sarah Gadel; Andrey A. Gorchakov; Tingting Gu; Peter V. Kharchenko; Samantha Kuan; Isabel Latorre; Daniela Linder-Basso; Ying Luu; Queminh Ngo; Marc Perry; Andreas Rechtsteiner; Nicole C. Riddle; Yuri B. Schwartz; Gregory A. Shanower; Anne Vielle; Julie Ahringer; Sarah C. R. Elgin; Mitzi I. Kuroda; Vincenzo Pirrotta; Bing Ren; Susan Strome; Peter J. Park; Gary H. Karpen; R. David Hawkins; Jason D. Lieb

doi:10.1038/nsmb.1972

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An assessment of histone-modification antibody quality

Journal article

Peer reviewed

An assessment of histone-modification antibody quality

Thea A. Egelhofer, Aki Minoda, Sarit Klugman, Kyungjoon Lee, Paulina Kolasinska-Zwierz, Artyom A. Alekseyenko, Ming-Sin Cheung, Daniel S. Day, Sarah Gadel, Andrey A. Gorchakov, …

Nature structural & molecular biology, Vol.18(1), pp.91-93

01/01/2011

DOI: https://doi.org/10.1038/nsmb.1972

PMCID: PMC3017233

PMID: 21131980

Abstract

Biochemistry & Molecular Biology

Biophysics

Cell Biology

Life Sciences & Biomedicine

Science & Technology

We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).

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Title: An assessment of histone-modification antibody quality
Creators - without role: Thea A. Egelhofer - University of California, Santa Cruz
Aki Minoda - University of California, Berkeley
Sarit Klugman - Ludwig Cancer Research
Kyungjoon Lee - Center for Systems Biology
Paulina Kolasinska-Zwierz - University of Cambridge
Artyom A. Alekseyenko - Brigham and Women's Hospital
Ming-Sin Cheung - Wellcome/Cancer Research UK Gurdon Institute
Daniel S. Day - Center for Systems Biology
Sarah Gadel - Washington University in St. Louis
Andrey A. Gorchakov - Brigham and Women's Hospital
Tingting Gu - Washington University in St. Louis
Peter V. Kharchenko - Center for Systems Biology
Samantha Kuan - Ludwig Cancer Research
Isabel Latorre - Wellcome/Cancer Research UK Gurdon Institute
Daniela Linder-Basso - Rutgers, The State University of New Jersey
Ying Luu - Ludwig Cancer Research
Queminh Ngo - Ludwig Cancer Research
Marc Perry - Ontario Institute for Cancer Research
Andreas Rechtsteiner - University of California, Santa Cruz
Nicole C. Riddle - Washington University in St. Louis
Yuri B. Schwartz - Rutgers, The State University of New Jersey
Gregory A. Shanower - Rutgers, The State University of New Jersey
Anne Vielle - University of Cambridge
Julie Ahringer - Wellcome/Cancer Research UK Gurdon Institute
Sarah C. R. Elgin - Washington University in St. Louis
Mitzi I. Kuroda - Brigham and Women's Hospital
Vincenzo Pirrotta - Rutgers, The State University of New Jersey
Bing Ren - Ludwig Cancer Research
Susan Strome - University of California, Santa Cruz
Peter J. Park - Center for Systems Biology
Gary H. Karpen - University of California, Berkeley
R. David Hawkins - Ludwig Cancer Research
Jason D. Lieb - University of North Carolina at Chapel Hill
Publication Details: Nature structural & molecular biology, Vol.18(1), pp.91-93
Publisher: Springer Nature
Number of pages: 4
Grant note: U01HG004258 / NATIONAL HUMAN GENOME RESEARCH INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Human Genome Research Institute (NHGRI) U01HG004270; U01HG004258 / US National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA U01ES017166 / Reference Epigenome Roadmap project U01ES017166 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
Identifiers: 9941315108331
Academic Unit: King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article