Abstract
In this paper, coenzyme Q(10) (Ubiquinone, CoQ(10)) was used for the first time as a transducer to construct electrochemical biosensor for effectively detecting gamma-L-glutamyl-L-cysteinyl-glycine (glutathione, GSH). CoQ(10) modified electrode was fabricated by attaching its gel mixed with multi-walled carbon nanotubes (MWNTs)/ionic liquid (IL). In the optimum conditions, based on the increasing of reduction peak current of CoQ(10) caused by GSH through voltammetric technology, it was found that the peak current of CoQ(10) was linear with the concentration of GSH in the range from 4.0 x 10(-9) to 2.0 x 10(-7) mol L-1 at the pH 7.00, and the limit of detection was 3.2 x 10(-10) mol L-1 (S/N=3). The results revealed that this method could be used to determine GSH in actual blood samples with the superiority of excellent selectivity, high stability and sensitivity. The strategy explored here might provide a new pathway to design novel multi-function transducers for detecting GSH, which has unique characteristic and potential application in the fields of sensor and medical diagnosis. (C) 2013 Elsevier B.V. All rights reserved.