Abstract
Excitation and emission spectra of Hantzsch reaction of aminocaproic acid.
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•Development of sensitive and rapid spectrofluorometric method for determination of 6-Aminocaproic acid.•Developed method based on the reaction of ethyl acetoacetate and formaldehyde with the primary amino group presented in 6-Aminocaproic acid.•The proposed method has been validated of according to the ICH guidelines.•The presented approach was adapted for in vitro study of 6-Aminocaproic acid in spiked human plasma and urine.
An innovative and sensitive spectrofluorimetric method has been developed for determination of 6-aminocaproic acid (ACA) in its pure form and its laboratory prepared tablets. The aim of this method is the reaction of ethyl acetoacetate and formaldehyde with the primary amino group presented in ACA as aimed in the Hantzsch reaction, this reaction resulted in formation of a yellow fluorescent dihydropyridine derivative that can be easily detected spectrofluorimetrically at 438 nm (excitation at 358 nm). At the optimum conditions of the reaction, the linear range was found to be (0.7–3.5 µg\mL) with limit of detection is 0.231 µg\mL and limit of quantitation is 0.700 µg\mL. The proposed method used for detection of ACA laboratory prepared tablets with average percentage 100.721 ± 0.701% without any interference from any excipients. This method used for in vitro determination of ACA in spiked human plasma with a percent mean recovery 99.874 ± 1.416%. In addition, the developed method used for determination of ACA in spiked human urine with percent mean recovery 100.314 ± 1.793%.