Abstract
Currently, the occurrence of toxic levels of metals in soils is a serious environmental issue worldwide. Phytoremediation is getting much attention to control metals soil pollution because it is economic and environmentally friendly. However, the methods used to detect metals in plants are not uniform and have depicted poor comparability of the research investigations. Therefore, the present overview is designed to discuss the possible chemical forms of metals in various environmental matrixes and the detection methods employed to identify the chemical forms of metals in plants. Moreover, the in situ and indirect methods to detect metals in plants have also been discussed herein. In addition, the pros and cons of the available techniques have also been critically analyzed and discussed. Finally, key points/challenges and future perspectives of these methods have been highlighted for the scientific community. Novelty statement In the current review, the possible chemical forms of metals in various environmental matrixes are discussed in detail. Various extraction agents and their efficiency for extracting metals from plants have been clearly illustrated. Further, all the available methods for analyzing the chemical forms of metals in plants have been compared.