Abstract
Nigella sativa (Black seed) is an annual flowering plant, belongs to the Ranunculaceae which has tremendous usage in medicine. In the treatment of digestive tract disorders, including gas, colic, diarrhea, dysentery, constipation, and hemorrhoids, the black seed is used. It is also used for asthma, allergies, cough, bronchitis, emphysema and other respiratory conditions. The seeds (50 g) were crushed, extracted with 700/0 methanol and filtered through filter paper. Antibacterial activity of the extract was assessed using the spread plate method against the Candida albicans. Different concentration of the extract i.e., 20, 40, 60, 80, 1000/o made and applied against mosquito larvae to determine the larvicidal activity of the extract. The results showed that the extract exhibited significant antimicrobial activity against C. albicans on the culture plate as compared to control. Different dilution showed larvicidal activity in a dose -dependent manner; however, 500/0 of larvicidal was noticed in all dilutions after 24 h. However, maximum activity was recorded in 60, 80, and 1000/0 dilutions after 72h. The results showed that the extract was extremely effective against the microbes even at the concentration of 500/0. Similarly, the extract was very effective against microbes and could be used as an antimicrobial agent.