Abstract
This study developed lipid nanocarriers, called squarticles, conjugated with anti-platelet-derived growth factor (PDGF)-receptor β antibody to determine whether targeted Minoxidil (MXD) delivery to the follicles and dermal papilla cells (DPCs) could be achieved. Squalene and hexadecyl palmitate (HP) were used as the matrix of the squarticles. The PDGF-squarticles showed a mean diameter and zeta potential of 195nm and −46mV, respectively. Nanoparticle encapsulation enhanced MXD porcine skin deposition from 0.11 to 0.23μg/mg. The antibody-conjugated nanoparticles ameliorated follicular uptake of MXD by 3-fold compared to that of the control solution in the in vivo mouse model. Both vertical and horizontal skin sections exhibited a wide distribution of nanoparticles in the follicles, epidermis, and deeper skin strata. The encapsulated MXD moderately elicited proliferation of DPCs and vascular endothelial growth factor (VEGF) expression. The active targeting of PDGF-squarticles may be advantageous to improving the limited success of alopecia therapy.
Topical use of minoxidil is only one of the very few treatment options for alopecia. Nonetheless, the current delivery method is far from ideal. In this article, the authors developed lipid nanocarriers with anti-platelet-derived growth factor receptor ? antibody to target dermal papilla cells, and showed enhanced uptake of minoxidil.
Selective delivery of minoxidil (MXD) to the hair follicles is important for treating alopecia with reduced adverse effect and systemic absorption. The final target of MXD delivery should reach the lower infundibulum where the dermal papilla cells (DPCs) reside. In this work, we chose the PDGF receptor as a target for MXD-loaded nanoparticles as it was capable of specifically delivering MXD to the DPCs in the hair follicles. The experimental results demonstrated that PDGF-squarticles are an active targeting nanocarrier that could largely deposit in the follicles and penetrate into the DPCs to induce proliferation and VEGF release. [Display omitted]