Abstract
The nanopolymers compositing and utilization for bioactive materials delivery could provide more bio-efficacy and biosafety targeting biological systems. Chitosan (CT) was extracted from black soldier "BSF; Hermetia illucens" fly and transformed to nanoparticles (NCT) via conjugation with gum Arabic (GA). Eugenol (Eug) and biosynthesized selenium nanoparticles (SeNPs) were innovatively loaded into NCT/GA nanocomposite and the entire composites were characterized and evaluated as antibacterial agents. The fabricated nanocomposites (SeNPs, NCT/GA, NCT/GA/Eug and NCT/GA/Eug/SeNPs) had mean particles sized of 9.7, 124.8, 132.6 and 134.2 nm, respectively, and the entire polymers nanocomposites carried positive charges of >+30 mV. The bacterial killing actions of fabricated polymers nanocomposites (NCT/GA) and their conjugates with Eug and SeNPs were assessed qualitatively and quantitatively and confirmed through scanning microscope imaging against Gram+ (Escherichia coli) and Gram -(Staphylococcus aureus) bacteria. Results indicated the forceful action of NCT/GA/Eug/SeNPs nanocompos-ites toward both strains, which significantly exceeded the standard antibiotic. The treated bacteria with NCT/GA/Eug/SeNPs had vigorous deformations and distortions in structures after 5 h and were com-pletely lysed/exploded after 10 h of exposure. The outcomes could recommend the applications of inno-vatively synthesized NCT/GA/Eug/SeNPs as powerful antimicrobial nanocomposite for potential usage in food and pharmaceutical sectors. (c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).