Abstract
Antibiotic resistance is one of the biggest hazards globally that is leading to prolonged hospital stay, inflated medical expenditures and increased morbidity and mortality. Many natural compounds have intrinsic antibacterial activity which needs to be exploited for their clinical use. Given its nutritional value, Date palm is considered as an essential nourishing source in many countries while the Date seed beverage is consumed locally to promote the health. The aim of our research is to assess the active constituents from date palm seed cake for their antimicrobial activity. Extraction of the date seed powder using high polarity solvents i.e. hexane and ethyl-acetate using cold extraction method. The isolates were later assessed for antibacterial activity against Gram-positive and Gram-negative bacteria using agar-well diffusion method. Theethyl-acetate extract in a 10% Dimethyl sulfoxide exhibited effective inhibition of bacterial growth in comparison with gentamycin. Potential antibacterial activity of ethyl acetate extract was observed against numerous Gram positive pathogens.