Abstract
The present study aims at biosynthesizing, characterizing and evaluating the biogenic silver nanoparticles (AgNPs) as antimicrobial and antibiofilm against Kocuria rosea and Kocuria rhizophila. Cellfree supernatant of Proteus mirabilis culture was used for biosynthesizing AgNPs, which confirmed by visualizing color change and X-ray diffraction. Transmission electron microscopy showed the formation of AgNPs in the range of 5-40 nm. ART-FTIR spectra provided evidence for presence of proteins as possible biomolecules responsible for stability of AgNPs and act as capping agent. AgNPs had ability to inhibit growth of K. rosea and K. rhizophila. The minimum inhibitory concentration (MIC90) of AgNPs against both strains was 25 mu g/mL. Antiadhesive effect of AgNPs was verified at sub-MIC90 dose (12.5 mu g/mL). The AgNPs concentrations up to 100 mu g/mL were not effective for complete removing the already established biofilms with maximum removing percentage of 30.5-34.9%. In conclusion, the present study demonstrated an unprecedented green process for biosynthesizing stable spherical-shaped AgNPs. Early control is suggested by preventing biofilm formation using low AgNPs concentration (12.5 mu g/mL) as a potential ingredient for formulating effective chemical sanitizers.