Abstract
beta-Glucan-binding protein (f3GBP) is important for the rational expansion of molecular biology. Here, zinc oxide nanoparticle (ZnONP) was synthesized using fiGBP from the crab Scylla serrata (Ss-beta GBP-ZnONP). Ss-beta GBPZnONP was observed as a 100 kDa band on sodium dodecyl sulfate polyacrylamide gel and characterized with UV-vis spectroscopy at 350 nm. X-ray diffraction analysis displayed values consistent with those for zincite. Fourier transform infrared spectroscopy revealed the presence of functional groups, including amide, alcohol, alkane, alkyl halide, and alkene groups. The zeta potential ( - 5.36 mV) of these particles indicated their stability, and transmission electron microscopy revealed the presence of 50 nm nanocones. Ss-beta GBP-ZnONPs were tested at 100 mu g/mL against the gram-positive Enterococcus faecalis and gram-negative Pseudomanas aeruginosa using confocal laser scanning microscopy and the bacterial viability assay was also performed. The growth of MCF7 breast cancer cells was inhibited following treatment with 75 mu g/mL Ss-beta GBP-ZnONPs. Thus, Ss-beta GBPZnONPs have the ability to control the growth of pathogenic bacteria and inhibit the viability of MCF7 breast cancer cell lines.