Abstract
The aim of this study was to investigate cytokine release from oral keratinocytes and
fibroblasts in response to AM and shortened derivatives previously characterised in terms
of their antimicrobial activities. Cells were incubated with AM or its fragments
(residues 1-12, 1-21, 13-52, 16-21, 16-52, 22-52, 26-52, and 34-52), and culture supernatants
collected after 1, 2, 4, 8, and 24 hours. A time-dependant increase in production of interleukin1-
α
and interleukin 1-
β
from keratinocytes in response to all peptides was demonstrated. However,
exposure to fragments compared to whole AM resulted in reduced production of these
cytokines (60% mean reduction at 24 hours,
P
<
.001
). No consistent differences were shown
between the cytokine response elicited by antimicrobial and nonantimicrobial fragments.
The production of interleukin-6 and interleukin-8 did not change significantly with time or
peptide used. Fibroblast cells were relatively unresponsive to all treatments. This study
demonstrates that antimicrobial activity does not predict cytokine response to adrenomedullin
or its shortened derivatives.