Abstract
Acinetobacter baumannii
has emerged as a problematic multidrug-resistant (MDR) pathogen worldwide. The aim of this study was to characterize clinical isolates of
A. baumannii
from Jeddah, a coastal city in the western region of Saudi Arabia that has an ∼50% expatriate population. In this study, 135 nonrepetitive clinical isolates of
A. baumannii
were identified through MALDI-TOF and susceptibility was determined with VITEK-2 systems and broth microdilution method. Genotypic characterization of the isolates was performed by using M13 primer typing and polymerase chain reaction screening was performed for carbapenemase genes, insertion sequences, metallo-β-lactamases, and cephalosporinase genes. The isolates were recovered from heterogeneous clinical specimens, and the majority of the cases of
A. baumannii
infection were acquired in the hospital and predominantly involved patients who were older than 50 years. Total, 58.5% of the isolates were MDR, and 55.6% isolates were resistant to carbapenem antibiotics. Approximately half of the isolates were resistant to ceftazidime, and cefepime among the β-lactam antibiotics and ciprofloxacin from the quinolone group. The
bla
OXA-23-like
gene and IS
Aba1
upstream of
bla
OXA-23-like
were detected in 92% of the carbapenem-resistant isolates, while all carbapenem-resistant isolates were found to carry
bla
OXA-51-like,
and
bla
ADC
-type cephalosporinase gene. The
bla
IMP
gene was detected in 84% isolates, and two isolates carried the
bla
NDM-1
gene. Data demonstrate the coexistence of multiple carbapenem resistance determinants in
A. baumannii
from the western region of Saudi Arabia.