Abstract
Antioxidant activities of the leaves and fruits of Oxystelmaesculentum were evaluated to isolate the most active compounds. Ethyl acetate fraction of leaves exhibited to the highest antioxidant with IC50 of 37.5 mu g/mL followed by chloroform. Isolation and structure determination of ethyl acetate and chloroform fractions leads to identification of a new compound, methyl 3 beta,20 alpha-dihydroxytaraxastane-28-oate (1) along with five known metabolites, cholesterol (2), cholest-22-en-3-ol, (3 beta, 5 alpha,22E) (3), quercetin (4), lupeol (5), 24-methylenecycloartenol (6), querectine-3-O-alpha-rhannnoside.The structure elucidation was accomplished mainly by Nuclear Magnetic Resonance spectroscopic and Mass spectrometric methods.