Abstract
This work assessed the phenolic and flavonoid components and their antioxidant, antifungal, and antibacterial effects in the ethanolic extract of barberry leaf and roots. The antibactericidal activity of root and leaf extracts against pathogenic bacteria was tested using agar diffusion and microdilution broth production for the lowest inhibitory concentration (MIC).
Berberis vulgaris
root and leaf extracts inhibited
Staphylococcus aureus
ATCC9973,
Escherichia coli
HB101,
Staphylococcus enteritis
, and
Escherichia coli
Cip812. The disc assay technique was used to assess the bactericidal activity of the extracts versus both pathogenic Gram-positive and Gram-negative strains. Hydro alcoholic extract was more effective against bacterial than fungal strains. The results showed that
Berberis vulgaris
leaf and roots extract had similar antifungal activities.
Berberis vulgaris
root extract inhibited the mycelial growth of
Penicillium verrucosum, Fusarium proliferatum
,
Aspergillus ochraceous, Aspergillus niger
, and
Aspergillus flavus
.
Berberis vulgaris
root extract has excellent antioxidant, antibacterial, and antifungal effects.
Berberis vulgaris
exhibited antimicrobial activity in vitro, and MIC showed that
Berberis vulgaris
parts efficiently affected pathogens in vitro. In conclusion, both
Berberis vulgaris
roots and leaves have considerable antibacterial activity and can be used as a source of antibacterial, antioxidant, and bioactive compounds to benefit human health.