Abstract
Endophytes associated with Calotropis procera and Phoenix dactylifera were screened for their capacity to produce bioactive metabolites against a panel of cancer cell lines and human pathogens. In the present study, the antiproliferative activity of ethyl acetate extract of MN05 displayed concentration-dependent inhibitory effects against the HepG2 and Jurkat cell lines, with an IC50 of 69.13 and 85.56 mu g/ml respectively. The extract induced morphological abnormalities in the cells and inhibited cells migration. Antimicrobial activity was found against both Gram positive and Gram negative microorganisms. No antifungal activity was detected against candida sp. MN05 extract showed very strong antibacterial effect against Mycobacterium sp. producing halos with an average diameter of 30.00 +/-.0000 mm followed by Bacillus cereus (23.0 +/- 00 mm), Bacillus subtilis (20.05 +/- 0.21) and Proteus vulgaris (19.50 +/- 0.07). MN05 extract enhanced IL-8 and IL6 secretions in HepG2 cells. Further investigation to identify the active compound/s and mode of action is required.