Abstract
Objective: Alternative medicine plays an important role today in searching for therapeutics for cancer and viral infection. So, a scientific validation to characterize constituents in the alternative medicines and therapeutic testing is warranted using modern instrumentation. Methods: In the present study, an old herbomineral formulation, KanthaRasavillai [KRV], was characterized using UV-vis spectrometry, FT-IR, XRD, SEM, and TEM study. Also, In vitro and in vivo studies were done to evaluate their antiviral and anticancer activity. FT-IR and XRD studies revealed a cocktail of nanoparticles of mercury, magnetic oxide, cinnabar, and arsenic. Results. Based on SEM, TEM, and XRD report, KRV contains nanoparticles in the size range of 9.1 nm to 25.0 nm. FT-IR analysis exposed the presence of several anti-cancerous bioactive compounds. Further in vitro testing against HCV virus proved KRV to inhibit HCV virus a close relative to SARS-CoV2. MTT assay confirmed the anticancer effect of KRV against Huh-7 and MCF-7 cell lines. Conclusion: The anticancer and antiviral properties in the ancient herbomineral drug with a cocktail of metal nanoparticles acknowledge the traditional medical practice as a pioneering approach for present-day ailments. However, the study concludes that the use of KRV depends on safety dosage and genuine preparation as described by ancient saints. (c) 2021 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).