Abstract
Verthimia iphionoides extract from Palestine was tested in vitro for its antioxidant, antibacterial, and anticancer activities. Total phenolic content (TPC) and total flavonoid content (TFC) measurements were made concurrently. By using FRAP and DPPH methods, the antioxidant activity were measured spectrophotometrically. By using HPLC-PDA, phenolic and flavonoid compounds of the extract were determined. Results showed strong antioxidant activity of the plant extract revealed by inhibition of stable free radicals (DPPH test) and strong reducing ability (FRAP test). According to spectrophotometric methods for total phenolic compounds and total flavonoids content, the extracts were also found to be rich in polyphenolic compounds and flavonoids. Verthimia iphionoides extract had high antibacterial activity against three bacterial strains (Escherichia coli, Staphylococcus aureus, and Streptococcus aureus), with inhibition zone values of 14 mm, 25 mm, and 27 mm, respectively. Bioactivities were primarily attributed to plants' abundant phenol-based chemical composition. Additionally, the extract was found to be abundant in phenolic and flavonoids, which improved its reducing activity and capacity to scavenge free radicals. Plant extracts were subjected to HPLC analysis, which identified different flavonoids and phenolic compounds in the extracts.