Abstract
Coronary artery disease (CAD) is one of the most common causes of morbidity and mortality worldwide. Genome wide association studies (GWAS) on Caucasian populations have revealed a major risk locus for CAD on chromosome 10p11.2. Therefore, we conducted an association study of the three most common SNPs reported on this locus (rs 2487928 A/G, rs2505083 C/T, rs 3739998 C/G) in a selected case control model to shed some light on the genetic factors associated with the occurrence of CAD in the Saudi population. A total of 1,004 chromosomes (500 chromosomes from Saudi CAD patients, who had experienced at least one myocardial infarction (MI) event and 504 chromosomes from Saudi age-matched healthy controls) were genotyped using real time PCR based TaqMan assay. Linkage disequilibrium (LD) analysis of three SNPs using haploview showed a significant difference in the genotype distribution for the SNP rs 2487928G between patients and controls (P = 0.00840, OR 1.4161, 95% CI 1.0930-1.8348, chi(2) 6.95). The other two SNPs, rs 3739998 and rs 2505083, were found to be CAD protective. Quality control of TaqMan results was carried out on 5% of the samples using Dye-terminator sequencing, the results of which concurred with the primary TaqMan genotypes. This study is in line with other studies that reported that the SNP rs2487928 G/G genotype located in KIAA1462 gene at 10p11.2 locus is significantly associated with CAD in other populations.