Abstract
Luffa plants showing symptoms of begomovirus infection were collected from Vehari, Punjab in 2016. RCA and PCR amplifications of begomovirus genomic components showed the presence of a typical Old World bipartite begomovirus
Tomato leaf curl New Delhi virus
(ToLCNDV). Interestingly,
Ageratum conyzoides
symptomless alphasatellite and CLCuMB were also found associated with bipartite begomoviruses in the same host, however, no monopartite begomovirus was detected. To the best of our knowledge, this is the first report of ToLCNDV with associated satellites infecting
L. cylindrica
in Pakistan. The importance of begomovirus extended host range with different combinations of associated satellites has been discussed.