Abstract
Osteogenesis is characterized by a serial of events involving cells attachment, proliferation, and differentiation. However, chitosan applications in osteogenesis mechanisms have remained limited. This study intends to examine chitosan's effect with different degrees of deacetylation (DDA) as a coating material for the Resorbable Blast Textured (RBT) implant surface. 63 Resorbable Blast Textured discs were coated either with 80 or 95 DDA. These discs were categorized into three groups i.e., RBT 80, RBT 95, and RBT control (without coating). After their separate applications, Cell viability, morphology, and bone formation were studied at 7 and 14 days. All samples showed biocompatibility and allowed cell attachment. However, areas with high cellular density were found in abundance around surfaces coated with chitosan in comparison with the control. At day 14, test groups coated with chitosan, especially 95 DDA, showed significant mineralization process and growth of nodule-like structures of hMSCs on the surfaces. No significant differences were found in cell viability except for RBT 80, which was lower in comparison to other groups. RBT 95 showed a significant increase in all osteoblast markers in comparison with RBT 80 and control. Chitosan material was confirmed as a right candidate for implant coated with Resorbable Blast Textured surface.