Abstract
•Spectrofluorimetric method for determination of three quinolones drugs Ofloxacin (OFX), Levofloxacin (LVX), and Pefloxacin (PFX).•The cited drugs reacted with Al+3 to produce highly fluorescent stable complexes The fluorescence intensity of the produced complex could be enhanced by the addition of sodium dodecyl sulfate (SDS).•Factors affecting fluorescence intensity (FI) were cautiously examined and adjusted.•Validation of analytical methods according to ICH and USP XXV validation guidelines was done.•The method was capable for the estimation of the investigated drugs both in-vitro as well as in vivo human plasma.
Simple, sensitive, rapid and specific spectroftuorimetric procedure has been described for the analysis of fluoroquinolones namely Ofloxacin (OFX), Levofloxacin (LVX) and Pefloxacin (PFX) in Pharmaceuticals and biological fluids. The named drugs reacted with Al+3 to produce highly fluorescent stable complexes measured at λem ranged from 436 to 480 nm (λex 329–333 nm). The fluorescence intensity of the produced complex could be enhanced by the addition of sodium dodecyl sulfate. The method had a good linearity within the concentrations ranged from 8.0 to 140.0 ng mL−1 for the cited drugs. The detection limits of the proposed method were 2.6, 3.2 and 4.50 ng mL−1 for the studied drugs. Factors affecting the fluorescence intensity of the resultant chelates were cautiously examined and adjusted. The developed method was employed effectively on the analysis of commercially available dosage forms with excellent accuracy and precision. Furthermore, the method was capable for the in-vitro as well as in vivo estimation of the investigated drugs in human plasma. The luminescence mechanism of the system is also discussed in detail, and the stoichiometry of the chelate formation reaction was obtained by Job’s method.