Abstract
A highly sensitive automated sequential-injection chemiluminescence (SIA-CL) method for determination of glucosamine sulphate (GLS) was developed. The goal of the present work is the evaluation of the enhancement effect of the investigated drug glucosamine sulphate on the chemiluminescence reaction between luminol and H2O2 in alkaline medium of 1.0 x 10(-2) mol L-1 sodium hydroxide at pH 11. The experimental conditions affecting the CL reaction such as the sequence of the reagents, concentrations, flow rate and aspirated volumes of reactants were systematically investigated and optimized. Under optimum conditions 50 L of 1.0 x 10(-3) mol L-1 luminol, 30 L of a GLS test solution and 50 L of 1.0 x 10(-2) mol L-1 H2O2 were used and the luminescing zone was pushed into the detector at a flow rate 100 L s(-1). The proposed method recorded high sensitivity, accuracy and simplicity that could be clarified as linear concentration range 1.0-2000 ng mL(-1) with rectilinear part (r = 0.9992, n = 9) and limit of detection 0.3 ng mL(-1), along with relative standard deviation 1.3%. It was found that the developed method can be used directly to determine the investigated drug GLS in its pharmaceutical dosage forms and in spiked serum and urine by diluting the samples for a 1000 fold. The obtained results were statistically analyzed and compared with those obtained by the reported method.