Abstract
Aim: To determine the recent bacterial profile and antimicrobial resistance pattern of uropathogenic bacteria of Enterobacteriaceae.
Methods: We performed this study on a total number of 6,269 urine samples collected from various wards of the Children's Hospital Lahore. The sample processing, isolation and identification were carried out by different microbiological techniques. The antimicrobial resistance pattern was determined by using Kirby Bauer Disk Diffusion method (E-test for colistin sulphate) and reported according to CLSI guidelines. E.coli and Klebsiella pneumoniae (ATCC 25922 and 13883) used as media quality control (QC). The data was analysed through IBM SPSS v.23.
Results: A significant bacteriuria was detected in 389(6.3%) females and males patients with a proportion of 219(55.6%) and 175(44.6%), respectively. The main culprits of urinary tract infection (UTIs) belonged to the Enterobacteriaceae found in our study were E. coli 211(53.6%) and Klebsiella species 157(39.8%). More than 84% of the bacterial strains were resistant to all of the cephalosporin drugs, 85% co-trimoxazole, 84.0% pipemidic acid, 83.5% nalidixic acid, 80.2% norfloxacin, 77.9% ciprofloxacin and 72.0% tobramycin. A comparatively lower resistance level seen against amikacin (36.8%), fosfomycin (35.3%), nitrofurantoin (28.4%), imipenem (25.9%) and colistin sulphate (21.8%).
Conclusion: The isolation of a high number of E. colt and Klebsiella spp. shows the continued predominance of these bacterial strains as uropathogens. Multidrug resistance among the uropathogens is worrisome which left us with fewer treatment options.