Abstract
As opportunistic bacterium Acinetobacter baumannii has the capability to develop resistance against different types of antibiotics. The goal of the research was to isolate and molecularly identify antibiotic resistance pathogenic bacteria from hospital and its bio-control using Coleus forskohlii ethanolic extract. For this about 78 swab samples were collected from different areas of the Prince Meshari Bin Saud General Baljarshi Hospital. The pathogenic bacteria were molecularly identified using 16s rRNA sequencing. In addition, the resistance profile of the strains was checked using different antibiotics. These includes Tetracyline (T), Chloramphenicol (C), Penicillin G (PG), Streptomycin (S), Erythromycin (E), Fusidic Acid (FC), Oxacillin (OX), Novobiocin (NO), Gentamicin (GM), Ampicillin G (AG), Sulphatriad (ST), Colistin sulphate (CS), Cotrimoxazole (CM), Cephalothin (CO), Trimethoprim (TM), Sulphametthoxazole (SMX), Clindamycin (CD), and Tetracycline (T). Antibacterial activity was measured using the microtiter broth dilution method to determine the minimum inhibitory concentration (MIC), as well as the minimum bactericidal concentration (MBC). Approximately 112 bacterial strains were isolated from 78 swab samples. Out of these bacterial strains, eight were identified as pathogenic bacteria using 16s rRNA sequences. All of these eight strains were derived from the Genus Acinetobacter. Among them, two strains of Acinetobacter were resistant to 14 different antibiotics. These two bacteria were identified as Acinetobacter baumannii and Acinetobacter sp. These MDR strains have been used for antibacterial activity against plant extract. The results showed that the MIC of the extract against these pathogenic strains was approximately 4 mg. Therefore, it is concluded that the plant extract has the ability to kill MDR resistant Acinetobacter strains in an ecofriendly way.