Abstract
Acute pancreatitis is a life threatening inflammatory disorder of pancreas. Currently, there is no effective pharmacological therapy available for this disorder and they does not showed any effect on root cause of the disease. The objective of the study was to evaluate the protective role of berberine in Dibutyltin dichloride (DBTC) induced acute pancreatitis in Wistar albino rats. The rats were divided into 4 groups and each carrying 6 rats. Group 1 served as control, Group 2 served as DBTC control (6mg/kg., i.p), Group 3 and 4 received 50mg/kg and 100mg/kg of berberine orally for a period of 28 days. Blood samples were collected at 1st, 3rd day and 28th day from all groups for the estimation of serum amylase and lipase. The DBTC reported to have effect on both exocrine and endocrine portion of the pancreas. The serum blood glucose, liver and kidney function markers (SGOT, SGPT, ALP, bilirubin, creatinine and protein); oxidative stress parameters such as SOD, CAT, GSH, Nitrite, MDA and Protein carbonyl content were estimated in both pancreatic and liver homogenates; inflammatory mediators (IL6, TNF α and CRP) were also estimated in both pancreatic and liver homogenates at the end of 28 days treatment period. The histopathological study was conducted for pancreas and liver. The treatment with berberine showed significant reduction of fasting blood glucose and liver kidney markers in DBTC induced pancreatitis in rats. The antioxidant enzymes SOD, CAT and GSH were found to be elevated and oxidative stress parameters (Nitrite, MDA and Protein carbonyl content) were found to be reduced with treatment with berberine in DBTC induced pancreatitis in rats. The berberine normalizes the elevated inflammatory cytokines with DBTC induction. The histopathology study indicating that berberine ameliorated the necrosis of both pancreatic acini and hepatocytes in DBTC induced pancreatitis in rats. The possible protective action against acute pancreatitis could be the action of berberine to counteract the oxidative damage caused by radicals or may attenuates the inflammatory infiltration induced by DBTC. Hence the berberine could be a potential source of traditional drug for acute pancreatitis.
This is from the Experimental Biology 2019 Meeting. There is no full text article associated with this published in The FASEB Journal.