Abstract
Two bidesmosidic saponins were isolated from
Climacoptera obtusifolia and their structures were determined as glucosides of gypsogenin (
1) and hederagenin (
2), by spectroscopic methods. Compounds
3 and
4 were identified as a known flavonoids which showed strong inhibitory activity on neutrophil oxidative burst response. Compound
2 significantly suppressed (92%) the ROS generation on the mononuclear cells 50
μg/mL concentration.
Two bidesmosidic saponins were isolated from
Climacoptera obtusifolia (Chenopodiaceae) and their structures were determined as gypsogenin 3-
O-[β-
d-xylopyranosyl-(1
→
3)-β-
d-glucopyranoside]-28-
O-{β-
d-glucopyranosyl} ester (
1) and hederagenin 3-
O-[β-
d-xylopyranosyl-(1
→
3)-β-
d-glucopyranoside]-28-
O-[β-
d-glucopyranosyl} ester (
2), by spectroscopic methods. Two known compounds, isorhamnetin 3-
O-β-
d-glucopyranoside (
3), and isorhamnetin 3-
O-[α-
l-rhamnopyranosyl-(1
→
6)-β-
d-glucopyranoside (
4) were also isolated for the first time from this plant. Compounds
1–
4 were tested in various immunomodulatory assays. Compound
2 suppressed (92%) the reactive oxygen species (ROS) production on mononuclear cells in luminol-based chemiluminescence (CL) assay at a higher concentration (50
μg/mL). Compounds
3 and
4 demonstrated a strong inhibition on ROS production in the oxidative burst activity of whole blood, neutrophils, and mononuclear cells. Additionally compounds
3 and
4 also suppressed PHA T-cell proliferation with no cytotoxic effects.