Abstract
This study reports a potential biotechnical method of 17-beta-estradiol removal in aquatic systems. The different bacterial strains isolated from sewage water samples were Bacillus sp., Enterobacter sp. I, Enterobacter sp. II, Klebsiella sp., Aeromonas veronii and Aeromonas punctate. These isolates were characterized on the basis of a single PCR reaction from the 5 ' end of the PCR fragment to obtain>700bp of high-quality sequence. The adjoining lineages of each organism were obtained by BLAST searches. The identified bacterial species were incubated in a saline media under different concentrations of 17-beta-estradiol in both aerobic and anaerobic conditions. The concentrations of CO2 and 17-beta-estradiol were measured by GC and HPLC, respectively. The percentage degradations of 17-beta-estradiol in aerobic and anaerobic conditions were 100 and 91.56%, respectively, using Klebsiella and Bacillus species. The developed and reported method is an inexpensive, selective and economic. This method may be used to remove 17-beta-estradiol in any water resource.