Abstract
This research was designed to evaluate the AgNPs synthesizing potential of methanol leaf extract of Justicia adhatoda and assessed their potential against multi-drug resistant microbes and in anticancer activity. The results obtained from this study showed that the methanol leaf extract of J. adhatoda has the potential to synthesized AgNPs, and it was initially confirmed by color (brown) change and observed sharp peak at 410 nm under UV-vis analysis. The FTIR analysis confirmed that the presence of functional groups involved in the fabrication of AgNPs. The XRD and EDX analyses confirm the crystalline structure and elemental composition of biofabricated AgNPs. The SEM and TEM analyses confirmed that the synthesized AgNPs were spherical and 5 to 20 nm in size. Furthermore, the synthesized AgNPs have outstanding antimicrobial activities against multi-drug resistant bacteria (S. pyrogens, E. coli, V. cholerae, S. aureus, and K. pneumoniae) and fungi (Penicillium sp., A. niger, C. albicans, Rhizobus sp., and A. flavus). Interestingly, the AgNPs showed remarkable anticancer activity (82.4%) against the cervical cancer cell line (HeLa) at the concentration of 100 mu g mL(-1) and LD50 value was found as 80 mu g mL(-1). These results concludes that J. adhatoda has the potential to synthesize AgNPs has remarkable biomedical applications.