Abstract
•Trichoderma sp., showed an abilities to synthesis of AgNPs and AuNPs with an excellent stability.•AuNPs significantly enhanced the bioelectricity production by MFC of anaerobic fermentation as catalyst.•Maximum bioelectricity production was optimized and obtained the voltage of 432.80mA using RSM.
The present work aimed to use the biogenic metallic nanoparticles as catalyst for bioelectricity production in microbial fuel cell (MFC) approach under anaerobic condition. Silver and gold nanoparticles (AuNPs) were synthesized using Trichoderma sp. Particle size and cystallinity were measured by X-ray diffraction revealed the crystalline structure with average size of 36.17 nm. Electron microscopic studies showed spherical shaped silver nanoparticles (AgNPs) and cubical shaped AuNPs with size ranges from 50 to 150nm. The concentration of biogenic metallic nanoparticles as catalyst for enhanced bioelectricity generations and estimated by response surface methodology (RSM) and found at the greatest of 342.80mA under optimized conditions are time interval, temperature, nanoparticles used as 63h, 28±2.0°C, 22.54mgl−1 (AgNPs) and 25.62mgl−1 (AuNPs) in a batch reactor. AuNPs acted as an excellent catalyst to enhance the bioelectricity production. This novel technique could be used for eco-friendly, economically feasible and facile electricity production.