Abstract
A NEW isolate of poty virus was isolated from naturally-infected henbane plants (Hyocyamus muticus L.) collected from the farm of Faculty of Pharmacy, Cairo University in January 2013. The naturally infected henbane plants exhibited severe mosaic, rugosity, blistering and malformation. Thermal inactivation point, dilution end point and longevity in vitro of virus isolate were found to be 65 degrees C, 10(-4) and 4 days, respectively. Electron micrograph of partial purified virus negatively stained with 2% phosphotungestic acid revealed the presence of filamentous viruses with size 1440 X14.3nm. Total RNA was extracted from infected henbane plant. Comparative nucleotide sequence analysis for virus showed a high degree of similarity (62 %) with four poty viruses accessions (EU482153: Potato virus Y isolate Foggia, KF850513: Potato virus Y isolate M3, AM184113: Henbane mosaic virus partial gene for poly protein-PHYS/H isolate, AY166867: Potato virus Y strain N isolate). The virus isolate was published in GenBank with accession number KM497011.