Abstract
In recent times, agricultural practices mainly rely on agrochemicals and pesticides to safe-guard edible crops against various pests and to ensure high yields. However, their indiscriminate use may cause severe environmental hazards that directly and negatively affect soil microorganisms and crop productivity. Considering these, present study was aimed to assess the toxicity of carbamate pesticides namely carbamoyl (CBL), methomyl (MML) and carbofuran (CBN) using bacterial and plant (Vigna mungo L.) bioassays. All pesticide doses (25–100 μg mL−1) showed negative effect on bacteria as well as plant. Growth, morphology, survival, cellular respiration and inner membrane permeability of Sinorhizobiumsaheli was hampered when exposed to pesticides. Pesticide induced morphological changes viz. aberrant margins; cellular cracking and distortion/damage in S. saheli were obvious under scanning electron microscope (SEM). The 100 μgCBNmL−1 had maximum inhibitory effect and it reduced survivability of S. saheli by 75%. In addition, biofilm formation ability of S. saheli was inhibited in a pesticides-dose dependent manner and it was statistically (p ≤ 0.05) significant. Pesticides indorsed significant changes in biomarker enzymatic assays and oxidative stress parameters towards S. saheli. Furthermore, at 100 μgCBNmL−1, germination efficiency, root, shoot length, plant survival and tolerance index of V. mungo were decrease by 50, 75, 65, 70 and 66%, respectively over control. Staining of pesticide treated roots with fluorescently labeled dyes propidium iodide (PI) and acridine orange (AO) showed increased oxidative stress, ROS generation and membrane permeability as revealed under confocal laser scanning microscope (CLSM). Furthermore, stressor metabolites and antioxidant enzymes in plant seedlings were progressively enhanced with increasing concentration of pesticides. Conclusively, present finding bestow an insights into a mechanistic approach of carbamate pesticide induced phyto, morpho and cellular toxic effects towards soil bacterium as well as plant with forthcoming implications for designing the pesticides to reduce their toxic/harmful effects.
•Pesticides affected growth, cellular viability, respiration, biofilms and inner membrane permeability of S. saheli RC10.•Pesticides reduced bacterial synthesis of indole3-acetic acids, siderophore and extra polymeric substances.•Exposure to excess concentration of pesticides induces oxidative damage via generation of ROS in V. mungo.•Pesticides decreased germination efficiency and growth of V. mungo.•H2O2 content, electrolyte leakage and antioxidant enzymes in plants were increased.