Abstract
In this study, green and eco-friendly biosynthesis of selenium nanoparticles (Se-NPs) were performed using
Penicillium expansum
ATTC 36200 for multiple biomedical applications. Mycosynthesized Se-NPs were completely characterized using UV, FT-IR, XRD, SEM, and TEM techniques. Se-NPs biosynthesized by
P. expansum
was characterized as a spherical shape with average size 4 to 12.7 nm. Moreover, Se-NPs were evaluated for multiple biomedical applications as antimicrobial, antioxidant, and anticancer activities and hemocompatibility. Results illustrated that Se-NPs have potential antimicrobial activity against Gram-positive (
Bacillus subtilis
ATCC6051 and
Staphylococcus aureus
ATCC23235), Gram-negative bacteria (
Escherichia coli
ATCC8739and
Pseudomonas aeruginosa
ATCC9027), fungi (
Candida albicans
ATCC90028,
Aspergillus niger
RCMB 02724 and
Aspergillus fumigatus
RCMB 02568), and antioxidant activity. Additionally, Se-NPs exhibited anticancer activity against PC3 cell line; IC
50
was 99.25 μg/mL. Meanwhile, they showed non-hemolytic activity on human RBCs at concentration up to 250 μg/mL. In conclusion, biosynthetic Se-NPs by
P. expansum
are promising for many safe-use biomedical applications.