Abstract
The utilization of green matrix on the manufacturing of nanocomposites has gained enormous interest in researchers to optimize an eco-friendly, cost-effective protocol in silver nanoparticles (AgNPs) production. AgNPs are among the most potent diagnostic and therapeutic representatives in the treatment of many diseases in nanomedicine field. In this research work, we focused on characterizations, antimicrobial, cytotoxic activity and apoptosis assessment against Pa-1 (Human ovarian teratocarcinoma) cell line via flow cytometry studies of biomodulated AgNPs using Cucumis sativus var. hardwickii fruit extract. The UV-Visible spectrum result revealed a maximum surface plasmon resonance at 408 nm. The characteristic FTIR analysis showed probable bioactive constituents subjected to reduction of silver ions. The morphology of Cs-AgNPs was spherical, polydispersed and size of the particles found to be 11.12 to 39 nm in range. In addition, the antimicrobial potential of Cs-AgNPs has shown moderate result against tested pathogens. Later, the biosynthesized Cs-AgNPs were tested for in vitro cytotoxic effect against Pa-1 cell line. The results revealed that the IC50 value of Cs-AgNPs against Pa-1 cells was observed to be 49.71 mu g/mL. In addition, apoptotic property was analyzed using Annexin V/Propidium iodide (PI) via flow cytometry technique. The obtained results were displayed significant early apoptosis cell population of 12.91% and late apoptosis cell population of 45.03% towards Pa-1 cell line, respectively. Thus, it can be ascertained that the biomodulated Cs-AgNPs possess significant cytotoxic effect against tested cell line. So, it will be useful aspect in medical field in future.