Abstract
This work embraced a systematic search for potentiated methodology of peptide synthesis through an approach for convenient synthesis of the nonapeptide (B-22-B-30) of insulin B-chain. The modified solid phase peptide synthesis (SPPS) with and without the application of microwave irradiation technique was used for synthesis of nonapeptide (B-22-B-30) of insulin B-chain with comparable study between the results of two methods. The produced target peptide was characterized by means of their amino acid analysis; Electrospray ionization mass spectroscopy (ESI-MS) and FT-IR data. Additionally, thermal gravimetric analysis (TGA) and differential thermal analysis (DTA) were used in the conformational study for prepared peptides. The results showed and confirmed all the advantages and importance of microwave irradiation technique in the peptide chemistry field, purity of the products, reducing reaction time and higher yield without any effect on the chemical structures and peptide bonds of the target peptide.