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CONSTRUCTION AND CHARACTERIZATION OF A BACTERIAL ARTIFICIAL CHROMOSOME LIBRARY OF ARABIDOPSIS-THALIANA
Journal article   Peer reviewed

CONSTRUCTION AND CHARACTERIZATION OF A BACTERIAL ARTIFICIAL CHROMOSOME LIBRARY OF ARABIDOPSIS-THALIANA

S Choi, R A Creelman, J E Mullet and R A Wing
Plant molecular biology reporter, Vol.13(2), pp.124-128
01/06/1995
DOI: https://doi.org/10.1007/BF02668782

Abstract

Biochemical Research Methods Biochemistry & Molecular Biology Life Sciences & Biomedicine Plant Sciences Science & Technology
We constructed an ordered 3,948-clone arabidopsis BAC Library. The library has a combined average insert size of 100 kb (n = 54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library.

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