Abstract
Mastitis is a complex and multifarious disease that adversely affects the physico-chemical properties, composition and yield of milk and risks of premature culling of affected animals A total of 320 Cholistani cattle were screened for subclinical mastitis using California mastitis test in and around district Bahawalpur. The blood and milk samples were only collected from subclinical infected cattle. Milk samples obtained from cattle with subclinical infection revealed significantly (P<0.05) negative correlation of Na with K, Ca, Mg, P, Fe and Zn while significant (P<0.05) positive correlation with aspartate aminotransferase, lactate dehydrogenase and alkaline phosphatase Serum samples from infected animals revealed significant (P<0.05) positive correlation of Ca with P and Zn while significant negative correlation of Zn and P with enzymes. Milk samples from non-infected cows showed a significant (P<0.05) negative correlation between Ca and K while positive correlations between Ca and P. Blood samples from non-infected animals revealed significant positive correlation between zinc, AST and ALP while significant (P<0.05) positive correlation between LDH, ALP and AST. From the present study, it was concluded that correlation of milk and blood electrolytes, trace minerals and enzymes are suitable diagnostic technique to detect sub-clinical mastitis.