Calcium-induced conformational changes in the amino-terminal half of gelsolin

Claude Roustan; Imen Ferjani; Sutherland K. Maciver; Abdellatif Fattoum; Bertrand Rebière; Yves Benyamin

doi:10.1016/j.febslet.2007.01.031

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Calcium-induced conformational changes in the amino-terminal half of gelsolin

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Calcium-induced conformational changes in the amino-terminal half of gelsolin

Claude Roustan, Imen Ferjani, Sutherland K. Maciver, Abdellatif Fattoum, Bertrand Rebière and Yves Benyamin

FEBS letters, Vol.581(4), pp.681-686

20/02/2007

DOI: https://doi.org/10.1016/j.febslet.2007.01.031

PMID: 17258204

Abstract

Actin cytoskeleton

Apoptosis

Calcium-binding

Gelsolin

Gelsolin is an actin-binding protein that is regulated by the occupancy of multiple calcium-binding sites. We have studied calcium induced conformational changes in the G1–2 and G1–3 sub-domains, and report the binding affinities for the three type II sites. A new probe for G3 has been produced and a K d of 5 μM has been measured for calcium in the context of G1–3. The two halves of gelsolin, G1–3 and G4–6 bind weakly with or without calcium, suggesting that once separated by apoptotic proteolysis, G1–3 and G4–6 remain apart allowing G1–3 to sever actin in a calcium free manner.

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Title: Calcium-induced conformational changes in the amino-terminal half of gelsolin
Creators - without role: Claude Roustan - UMR 5539 (CNRS) Laboratoire de motilité cellulaire (Ecole Pratique des Hautes Etudes), Université de Montpellier 2, Place E. Bataillon, CC107, 34095 Montpellier Cedex 5, France
Imen Ferjani - UMR 5539 (CNRS) Laboratoire de motilité cellulaire (Ecole Pratique des Hautes Etudes), Université de Montpellier 2, Place E. Bataillon, CC107, 34095 Montpellier Cedex 5, France
Sutherland K. Maciver - University of Edinburgh
Abdellatif Fattoum - Centre de Recherches de Biochimie Macromoléculaire, FRE 2593 (CNRS), 1919 rte de Mende, 34293 Montpellier Cedex 5, France
Bertrand Rebière - Centre de Recherches de Biochimie Macromoléculaire, FRE 2593 (CNRS), 1919 rte de Mende, 34293 Montpellier Cedex 5, France
Yves Benyamin - UMR 5539 (CNRS) Laboratoire de motilité cellulaire (Ecole Pratique des Hautes Etudes), Université de Montpellier 2, Place E. Bataillon, CC107, 34095 Montpellier Cedex 5, France
Publication Details: FEBS letters, Vol.581(4), pp.681-686
Publisher: Elsevier B.V
Identifiers: 9915466108331
Academic Unit: Imam Abdulrahman Bin Faisal University
Language: English
Resource Type: Journal article