Abstract
This study determined the full-length sequence of
CYP2E1
, one of six cytochrome P450 genes previously examined in camel tissues by western blotting and semi-quantitative PCR. The
Camelus dromedarius CYP2E1
has an open reading frame of 1,473 bp, and the cDNA encodes a protein of 490 amino acid residues with a molecular weight of 54.8 kDa. The deduced amino acid sequence showed the highest identity with
Bos taurus
(88%),
Sus scrofa
(87%), and
Homo sapiens
(83%). In a phylogenetic analysis, the
C. dromedarius
CYP2E1 isoform was located beside cattle and pigs. The deduced amino acid sequence of camel CYP2E1 showed the conserved proline-rich amino terminus and the heme-binding signature localized near the carboxy terminus of the protein.