Abstract
The induction of α- and β-galactosidases in six strains of
Lactobacillus reuteri (
L.
reuteri) by six carbohydrate sources and four protein sources was studied.
L.
reuteri grown on raffinose had the highest α-galactosidase activity (10.55 Gal
U/ml), while lactose exhibited the highest β-galactosidase activity (43.82 Gal
U/ml) when compared to other carbohydrate sources.
L.
reuteri grown on yeast extract exhibited the highest α- and β-galactosidases activity (15.27 and 12.88 Gal
U/ml, respectively) when compared to other protein sources. MF14C and SD2112 grown on raffinose had the highest α-galactosidase activity (14.75 and 14.18 Gal
U/ml, respectively) followed by CF2-7F (13.38 Gal
U/ml). CF2-7F grown on lactose had the highest β-galactosidase activity (82.01 Gal
U/ml). SD2112, MM2-3 and CF2-7F grown on yeast extract (20.96, 19.67, 19.67 Gal
U/ml, respectively) showed the highest α-galactosidase activity. MM2-3 and CF2-7F grown on yeast extract showed the highest β-galactosidase activity (18.1 and 17.59 Gal
U/ml, respectively). Raffinose and lactose were the best carbohydrate sources to produce α- and β-galactosidases, respectively. Yeast extract was the best protein source to produce both enzymes and CF2-7F strain was the best producing strain on all tested conditions.