Abstract
This study succeeded in the isolation of potent laccase endophytic fungus producer Alternaria arborescens MK629314 from medicinal plants collected from Wadi Abu Matir, Saint- Catherine-Protectorate, South Sinai-Egypt. Full physiochemical and thermodynamic characterization for A. arborescens MK629314 laccase was investigated showing the highest activity at a temperature of 50 degrees C and pH 5. The kinetics constants, K-m (Michael's constant) and V-max (maximum velocity) were determined to be 4 mM and 133.33 U.ml(-1), respectively. Also, the thermodynamics E-a (activation energy) and E-d (activation energy of denaturation) were determined to be 15.13 and 48.85 kJ.mol(-1), respectively. Thermal denaturation parameters as K-d (denaturation rate constant), T-1/2 (half-life), D-values (decimal reduction time), Delta H-o (enthalpy), Delta G(o) (Gibbs free energy), and Delta S-o (entropy) were determined. The pretreatment of olive mill wastewater (OMWW) 10 and 20% with A.arborescens MK629314 caused, the maximum polyphenols removal of 63.06 and 27.29%, respectively. A.arborescens MK629314 of 50% acetone fraction succeeded in saccharification of five agricultural wastes with the highest reducing sugar recovery from cantaloupe peel which was improved by central composite design to be 240.90 mg.ml(-1). A. arborescens MK629314 and its produced laccase proved to be a biological sufficient tool for the detoxification of wastewater from the olive oil industry and delignification of wastes from agriculture.
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