Abstract
The aims of the present study were to test the antibacterial activity and chemical composition of Trigonella foenum-graecum extract. The extract was obtained using 50% aqueous - ethanol extraction solution to extract T. foenum-graecum seeds. The extract was prepared and evaluated for antimicrobial activity against six bacterial strains by determining minimum inhibitory concentration (MIC). The results revealed that the 50% aqueous - ethanol extract is potent in inhibiting bacterial growth of both gram-positive and gram negative bacteria. The chemical composition of fenugreek was analyzed by gas chromatography/mass spectroscopy (GC/MS). The hydroxymethyl furfural, gingerone, alpha-curcumene, bergamotene, and gingerol were the highest abundant compounds out of total 31 compounds were identified in the fenugreek extract.