Abstract
The present study was carried out to analyze chemical constituents and antibacterial activity of ethanolic leaf extract of
Cardiospermum halicacabum
(ECH). The FT-IR spectrum confirmed the presence of alcohols, phenols, alkanes, alkynes, aliphatic ester and flavonoids in ECH. The GC–MS analysis revealed that ECH contained about twenty four compounds. The major chemical compounds identified were cyclohexane-1, 4, 5-triol-3-one-1-carboxylic acid, benzene acetic acid, caryophyllene, phytol and neophytadiene. The ECH was screened for its antibacterial activity against different bacterial strains and anti fungal activity against
Candida albicans
by agar well diffusion and minimum inhibitory concentration (MIC) assay. ECH exhibited antibacterial and antifungal activity. All the tested bacterial strains showed MIC values ranging from 80 to 125 μg of extract/ml and
C. albicans
showed 190 μg of extract/ml as a MIC. The maximum activity ECH was observed against human pathogen
Staphylococcus aureus
followed by
Escherichia coli
and the fish pathogen
Aeromonas hydrophila
. ECH exhibited moderate activity against some of the tested multidrug resistant strains.