Abstract
WOS: 000343058200005
Artemisia argyi, A. feddei, A. gmelinii, A. manshurica, and A. olgensis (Asteraceae) were collected in Far East Russia. Oils were hydrodistilled and simultaneously analyzed by GC-FID and GC/MS. Main constituents were found as follows in Artemisia oils: selin-11-en-4 alpha-ol (18.0%), 1,8-cineole (14.2.0%), artemisia alcohol (12.9%), borneol (9.7%) in A. argyi; camphor (31.2%), 1,8-cineole (17.6%), alpha-thujone (5.7%) in A. feddei; longiverbenone (12.0%), isopinocamphone (8.9%) 1,8-cineole (6.7%) camphor (5.8%), trans-p-menth-2-en-1-ol (5.3%) in A. gmelinii; germacrene D (11.2%), rosifoliol (10.1%), caryophyllene oxide (6.8%), eudesma-4(15), 7-dien-1 beta-ol (5.6%) in A. manshurica; eudesma-4(15), 7-dien-1b-ol (6.9%), caryophyllene oxide (5.6%), guaia-6,10(14)-dien-4 beta-ol (5.1%) and hexadecanoic acid (5.0%) in A. olgensis. Oils were subsequently submitted for antifungal and antimosquito evaluations. Artemisia species oils showed biting deterrent effects in Aedes aegypti and Artemisia gmelinii oil with the most active biting deterrence index values of 0.82 +/- 0.1 at 10 m g/mL. Larval bioassay of A. gmelinii and A. olgensis oils showed higher larvicidal activity against Ae. aegypti larvae with LD50 values of 83.8 (72.6-95.7) ppm and 91.0 (73.8-114.5) ppm, respectively. Antifungal activity was evaluated against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloeosporioides using direct overlay bioautography assay and all showed nonselective weak antifungal activity. Antioxidant evaluation of the oils was performed by using beta-carotene bleaching, Trolox equivalent and DPPH tests. The tested Artemisia oils demonstrated moderate antioxidant activity.
Deployed War-Fighter protection (DWFP) Research Program; U.S. Department of Defense through the Armed Forces Pest Management Board (AFPMB); USDA, ARS [56-6402-1-612]
This study was supported by a grant from the Deployed War-Fighter protection (DWFP) Research Program and the U.S. Department of Defense through the Armed Forces Pest Management Board (AFPMB) and USDA, ARS grant No. 56-6402-1-612. We thank Ms. J. L. Robertson and Ms. R. Pace for the antifungal bioassays and thank to Dr. James J. Becnel, Mosquito and Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, for supplying Ae. aegypti eggs.