Abstract
In the present investigation, extracts obtained from L. guyonianum Durieu ex Boiss. aerial parts were used to evaluate total phenolic, flavonoid and tannin contents. A study of antioxidant activities of the prepared samples was carried out on the basis of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2-2'-azino-bis-(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS+.) and ferric reducing antioxidant power (FRAP) assays. Moreover, the efficiency of methanolic, chloroformic and petroleum ether extracts and the deriving fractions from the methanolic extract was tested against human bacterial and fungal pathogenic strains using micro dilution method in 96 multiwell microtiter plate. Furthermore, leaves and stems extracts were subjected to RP-HPLC for phenolic compounds identification. Results showed that polyphenolic contents and antioxidant activities varied considerably as function of solvent polarity. Moreover, antiradical capacities against DPPH, ABTS(+.) and reducing power were maxima in methanol aerial parts extract which showed the highest polyphenol contents (134mg CE/g DW). The antimicrobial activities showed that methanolic, chloroformic and petroleum ether extracts were found to be most potent against Pseudomonas aeruginosa and Staphylococcus aureus with MIC values of 23 and 46μ.mL(-1), respectively. The fractions F(13) and F(16) have a great antifungal potential against Candida glabrata, Candida krusei and Candida parapsilesis (MIC=39μ.mL(-1)). The RP-HPLC analysis lead the identification of gallic, procatechuic and trans-cinnamic acids, methyl-4-hydroxybenzoate, n-propyl-3,4,5-trihydroxybenzoate, epicatechin, naringin and myricetin in L. guyonianum Durieu ex Boiss. leaves and stems extracts.