Abstract
In an attempt to reduce the massive application of the toxic chemical pesticides, essential oils (EOs) of Achillea biebersteinii and Juniperus procera were obtained through hydrodistillation and analyzed using (GC–FID) and (GC–MS). α-terpinene and p-cymene were detected as the major components in the EO of A. biebersteinii, while eugenol and ß-caryophyllene were the major constituents in the EO of J. procera. The plant EOs and major fractions act as considerable mosquitocides against Aedes aegypti L, the common transmitter of Dengue fever. The EOs and major fractions were tested at 6.25, 12.5, 25, 50 and 100 μl/l. Insect mortality was time and dose-dependent, and the adult stage was more sensitive than larvae. At a concentration of 50 μl/l, 24 post treatment larval and adult mortality ranged between (40.3 and 89.3%) and (51.4 and 95.6%), respectively. The LC50 values ranged between 12.2 and 70.1 μl/l against larvae and between 10.1 and 63.12 μl/l against adults. All of the crude EOs were more potent than their major fractions. Eugenol and ß-caryophyllene showed strong mosquitocidal activity than p-cymene and α-terpinene. The corrected percentage mortality was increased over time with all of the test materials. In terms of lethal time required to kill 50% of the population (LT50), a concentration of 100 μl/l of J. procera EO showed LT50 values of 2.3 and 1.7 h against larvae and adult, respectively. The EOs induced considerable inhibition of acetylcholinesterase activity, where J. procera crude oil (IC50 = 13.12mM) and eugenol (IC50 = 19.65mM) were the most potent. Results proved that the test plant oils and their major fractions could be developed as natural pest control agents to control A. aegypti.
Pesticide; Biological sciences; Ecology; Entomology; Insects; Achillea biebersteinii; Juniperus procera; Essential oils; Mosquitocidal activity; Aedes aegypti.