Abstract
Background: Unlike the terrestrial region, the microorganisms especially actinomycetes groups existing inthe marine environment are important sources for the medically important drugs and other active compounds. Considering the importance of natural compounds from the marine actinomycetes, the presentstudy proceeded to identify and characterize promising antibacterial and anticancer actinomycetes fromthe marine region of Saudi Arabia and to profile the individual chemical components. Methods: Antimicrobial, anticancer and chemical profiling were performed by broth microdilution, mitochondrial membrane potential assays and GC-MS analysis. Investigations were directed towards theisolation and characterization of active Streptomyces sp. strain Al-Dhabi-97. Results: The obtained results of the morphological, biochemical, physiological and molecular level studiesof the isolate Al-Dhabi-97 showed similarity towards the species of Streptomyces. Gram positive bacteriasuch as Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis and Staphylococcus aureus showedMIC values of 500, 250, 125 and 62.5 mu g/ml and Gram negative bacteria such as Pseudomonas aeruginosa, Klebsiella pneumonia, Escherichia coli and Salmonella paratyphi reported MIC values of 500, 500, 250 and> 250 mu g/ml in the antimicrobial studies. The results of anticancer studies showed that at 100 mu g/ml, the extract showed maximum cell growth inhibition and exhibited 2.5% necrosis, 62.2% late apoptosisand 20.8% early apoptosis in COLO 320 DM and VERO cell lines respectively. Chemical profiling of theextract authenticated the presence of constituents such as 1-phenanthrenemethanol (46.64%), phthalicacid, di(2-propylpentyl) ester (26.97%), benzenebutanoic acid (3.37%), podocarp-7-en-3-one (2.68%), andindole3-carboxaldehyde (1.11%) respectively. Conclusion: The present study concluded that Saudi Arabian marine region was a promising area for theidentification of medically important natural products producing actinomycetes for antibacterial andanticancer drugs. (c) 2019 The Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz Universityfor Health Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).