Abstract
The rabbit immunoglobulin antibodies (IgGs) have been immobilized onto nanobiocomposite film of chitosan (CH)–iron oxide (Fe
3O
4) nanoparticles prepared onto indium–tin oxide (ITO) electrode for detection of ochratoxin-A (OTA). Excellent film forming ability and availability of –NH
2 group in CH and affinity of surface charged Fe
3O
4 nanoparticles for oxygen support the immobilization of IgGs. Differential pulse voltammettry (DPV) studies indicate that Fe
3O
4 nanoparticles provide increased electroactive surface area for loading of IgGs and improved electron transport between IgGs and electrode. IgGs/CH–Fe
3O
4 nanobiocomposite/ITO immunoelectrode exhibits improved characteristics such as low detection limit (0.5
ng
dL
−1), fast response time (18
s) and high sensitivity (36
μA/ng
dL
−1
cm
−2) with respect to IgGs/CH/ITO immunoelectrode.