Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites

Yong H Woo; Hifzur Ansari; Thomas D Otto; Christen M Klinger; Martin Kolisko; Jan Michálek; Alka Saxena; Dhanasekaran Shanmugam; Annageldi Tayyrov; Alaguraj Veluchamy; Shahjahan Ali; Axel Bernal; Javier del Campo; Jaromír Cihlář; Pavel Flegontov; Sebastian G Gornik; Eva Hajdušková; Aleš Horák; Jan Janouškovec; Nicholas J Katris; Fred D Mast; Diego Miranda-Saavedra; Tobias Mourier; Raeece Naeem; Mridul Nair; Aswini K Panigrahi; Neil D Rawlings; Eriko Padron-Regalado; Abhinay Ramaprasad; Nadira Samad; Aleš Tomčala; Jon Wilkes; Daniel E Neafsey; Christian Doerig; Chris Bowler; Patrick J Keeling; David S Roos; Joel B Dacks; Thomas J Templeton; Ross F Waller; Julius Lukeš; Miroslav Oborník; Arnab Pain

doi:10.7554/eLife.06974

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Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites

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Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites

Yong H Woo, Hifzur Ansari, Thomas D Otto, Christen M Klinger, Martin Kolisko, Jan Michálek, Alka Saxena, Dhanasekaran Shanmugam, Annageldi Tayyrov, Alaguraj Veluchamy, …

eLife, Vol.4(2015), pp.e06974-41

15/07/2015

DOI: https://doi.org/10.7554/eLife.06974

PMCID: PMC4501334

PMID: 26175406

Abstract

Alveolata - genetics

DNA, Algal - chemistry

DNA, Algal - genetics

Evolution, Molecular

Gene Expression Profiling

Molecular Sequence Data

Sequence Analysis, DNA

The eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga.

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Title: Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites
Creators - without role: Yong H Woo - King Abdullah University of Science and Technology
Hifzur Ansari - King Abdullah University of Science and Technology
Thomas D Otto - Wellcome Sanger Institute
Christen M Klinger - University of Alberta
Martin Kolisko - Canadian Institute for Advanced Research
Jan Michálek - Biology Centre of the Czech Academy of Sciences
Alka Saxena - King Abdullah University of Science and Technology
Dhanasekaran Shanmugam - National Chemical Laboratory
Annageldi Tayyrov - King Abdullah University of Science and Technology
Alaguraj Veluchamy - Institut de Biologie de l'École Normale Supérieure
Shahjahan Ali - King Abdullah University of Science and Technology
Axel Bernal - University of Pennsylvania
Javier del Campo - Canadian Institute for Advanced Research
Jaromír Cihlář - Biology Centre of the Czech Academy of Sciences
Pavel Flegontov - Biology Centre of the Czech Academy of Sciences
Sebastian G Gornik - University of Melbourne
Eva Hajdušková - Biology Centre of the Czech Academy of Sciences
Aleš Horák - Biology Centre of the Czech Academy of Sciences
Jan Janouškovec - Canadian Institute for Advanced Research
Nicholas J Katris - University of Melbourne
Fred D Mast - Center for Infectious Disease Research
Diego Miranda-Saavedra - Centro de Biología Molecular Severo Ochoa
Tobias Mourier - University of Copenhagen
Raeece Naeem - King Abdullah University of Science and Technology
Mridul Nair - King Abdullah University of Science and Technology
Aswini K Panigrahi - King Abdullah University of Science and Technology
Neil D Rawlings - European Bioinformatics Institute
Eriko Padron-Regalado - King Abdullah University of Science and Technology
Abhinay Ramaprasad - King Abdullah University of Science and Technology
Nadira Samad - University of Melbourne
Aleš Tomčala - Biology Centre of the Czech Academy of Sciences
Jon Wilkes - Wellcome Centre for Molecular Parasitology
Daniel E Neafsey - Broad Institute
Christian Doerig - Monash University
Chris Bowler - Institut de Biologie de l'École Normale Supérieure
Patrick J Keeling - Canadian Institute for Advanced Research
David S Roos - University of Pennsylvania
Joel B Dacks - University of Alberta
Thomas J Templeton - Cornell University
Ross F Waller - University of Melbourne
Julius Lukeš - Biology Centre of the Czech Academy of Sciences
Miroslav Oborník - Biology Centre of the Czech Academy of Sciences
Arnab Pain - King Abdullah University of Science and Technology
Publication Details: eLife, Vol.4(2015), pp.e06974-41
Grant note: HHSN272200900018C / PHS HHS
Identifiers: 9921884408331
Academic Unit: King Saud Bin Abdulaziz University for Health Sciences; King Abdullah University of Science & Technology
Language: English
Resource Type: Journal article