Abstract
Genetically engineered bacteria for pollution control of heavy metal have been widely studied, however, using Pseudomonas aeruginosa (P. aeruginosa) that can adapt to various circumstances to remediate heavy metal pollution is rarely reported. In this study, we employed CadR, a cadmium (Cd)-specific binding protein, displaying on the surface of P. aeruginosa with chromosomal expression. The genetically engineered (GE) P. aeruginosa still flourished in the 30th generation in the LB broth which contained 100 μM Cd(II), exhibiting an excellent genetic stability. Chromosomally expressed P. aeruginosa showed an adsorption capacity of up to 131.9 μmol/g of Cd(II). In addition, the low concentration of the coexisting two valence ions has no significant effect on adsorption capacity of Cd(II). This study provides a direction for application of P. aeruginosa in environment remediation.
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•We first express CadR that is specific to cadmium on the surface of P. aeruginosa.•The chromosomal engineering P. aeruginosa exhibiting excellent adsorption capacity•The chromosomal engineering P. aeruginosa has great hereditary stability.•The engineering P. aeruginosa is expected to be used in practical applications.